Polyclonal secondary antibodies

The ideal tool for all immunochemistry assays

For all techniques

And all detection systems

Immunotitration EIA / ELISA
Chemiluminescence
Dot / Western Blot
Immunoenzymology
Immunohistochemistry
Immunofluorescence
Immunocytochemistry
Immunoprecipitation
Flow cytometry
Immunoseparation

Make your choice within a large range of antibodies

Various species Various specificities Various labeling Suitable qualities
anti-human Ig
anti-IgG, IgM, IgA
fluorescent
globulins
anti-mouse and rabbit Ig
anti-IgGAM
enzymatic
affinity purified antibodies
anti-goat, bovine, dog Ig ...
anti-Fc and Fab fragments
biotin
Fab and F(ab')2 fragments

Production steps

 

Abliance secondary antibodies are polyclonal antibodies, obtained after animal hyperimmunisation (rabbit, goat or sheep) with human or animal immunoglobulins. The collected immune sera contain, in addition to specific antibodies, others non-specific antibodies and serum proteins.
Globulins
After a succession of non-specific purifications (delipidation, ammonium sulphate precipitation and dialysis), globulins are obtained. This material is suitable for immuno-precipitation techniques.
Affinity purified antibodies
The antibodies are then purified by affinity chromatography, thus ensuring good specificity.
Moreover, the anti mouse and rabbit IgG (H+L) are systematically adsorbed onto a gel chromatography column with human IgA, G and M and so do not cross react with human IgGAM.
Fragments
For some applications (immuno-histology/cytology, flow cytometry) Abliance has available F(ab')² and Fab fragments obtained by enzymatic digestion. These fragments allow less non-specific binding than whole antibodies containing the Fc region. Smaller than whole antibodies, the fragments better enter into cells and tissues.
Labeled antibodies
Abliance also offers various kinds of conjugates: globulin fractions, purified antibodies and Fab or F(ab')² fragments are conjugated to fluorochromes (fluorescein, rhodamine), enzymes (peroxidase, alkaline phosphatase, beta-galactosidase, glucose oxidase) and biotin.