Purification, Conjugation, Fragmentation

Antibody conjugation

You can rely on our professionnal team to quickly and efficiently label your antibodies.

Our antibody labeling service is competitive with antibody labeling kits.

We cover all your needs:

  • Enzyme labeling (peroxydase, alcalin phosphatase, oxydase glucose, b-galactosidase)
  • Fluorescent labeling (Fluorescein (FITC), Rhodamine (TRITC), Cyanine, Phycoerythrin)
  • Biotynilations
  • Development of labeling protocol with all commercial labels

Antibody fragmentation

Antibody activity is supported by a small region that represents only 2% of the molecule. The rest of the molecule can lead to undesired effects :

  • in detection methods, the whole antibody can generate non-specific interactions (background signal)
  • for therapeutic use, the antibody has an immunogenicity that can cause anaphylactic shocks

The antibodies can be treated to obtain fragments F(ab')2 or Fab. The F(ab')2 fragments have a much better apparent affinity and hold their property of precipitation thanks to their bivalent form. The Fab fragments are monovalent. They are particularly recommended to hit hard-to-reach targets when molecule diffusion is a limiting factor.