Reagent for determination of Prothrombin time (PT) in human plasma
The method uses Quick and al. method.
Tissue thromboplastin and calcium are added to citrated plasma, activating the factors of extrinsic coagulation pathway. The clotting time (time to formation of a fibrin clot) is measured at 37°C.
The Prothrombin time is used as a screening test to detect disorders in the extrinsic coagulation pathway.
The results in seconds are converted into percentages to evaluate the prothrombinic activity in comparison to a reference plasma consider as 100%.
A deficient prothrombinic activity can occur in the following cases:
- Hepatic disease
- Hemorrhagic disease of new born
- Congenital factor deficiency (factors II, V, VII or X)
- Vitamin K deficiency
- Vitamin K antagonist treatment
- Circulating anticoagulants
- Disseminated intravascular coagulation (DIC)
The results in seconds are converted into INR (International Normalized Ratio) in the particular use of monitoring oral anticoagulant therapy (Vitamin K antagonists).
Presentation and Technical data sheet
|CODE||Presentation||Technical Data Sheet||MSDS|
|PT02||R1 : 5 x 2mL
R2 : 1 x 12mL (Reconstitution buffer)
|PT05||R1 : 10 x 5mL
R2 : 1 x 60mL (Reconstitution buffer)